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Recombinant A8R–A23R complex complements other components required for intermediate transcription. The following components were added to a transcription assay: vaccinia virus RNA polymerase (RNAP), extract of uninfected HeLa cells (HeLa), capping enzyme plus VITF-1-containing fraction from infected HeLa cells (CE + VITF-1), and the A8R–HisA23R complex purified from E. coli. Transcripts were analyzed as described in the legend to Fig. 1.
