Figure 6.

Northern blot analysis of mRNAs from cells infected with vaccinia virus. HeLa cells were infected with vaccinia virus in the absence (−) or presence (+) of AraC and harvested from 1 to 6 h later. In addition, uninfected HeLa cells (Un) and HeLa cells infected in the presence of cycloheximide (Chx) for 6 h were harvested. RNAs were resolved by electrophoresis on a denaturing agarose gel, transferred to a nylon membrane, and hybridized with ³²P-labeled DNA probes specific for the C11R, A8R, and A23R ORFs. Autoradiograms are shown.