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Analysis of Gag protein. (A) Radioimmunoprecipitation assay analysis of in vitro-transcribed and -translated Gag. Antiserum to Flag epitope was used. Lane 1, pCDNA3 vector; lane 2, P93A; lane 3, G94A; lane 4, Q95A; lane 5, M96A; lane 6; ΔPGQM. (B) Cell lysate. (C) Culture supernatant of HeLa cells transfected with Gag plasmid. Immunoblot analysis was used. Details as above.
