Figure 4.
Radioimmunoprecipitation assay analysis of viral proteins. RD cells were transfected with 10 μg of HIV-1 proviral DNA by using the calcium phosphate precipitation method. Cells were labeled with [35S]methionine for 12 hours. Both cells and culture supernatants were processed as described in Materials and Methods. (A) Cells. (B) Culture supernatant. Lane 1, NL-P93-A; lane 2, NL-G94A; lane 3, NL-Q95A; lane 4, NL-M96A; lane 5, NL-ΔPGQM; lane 6, NL4-3. Immunoprecipitation with normal sera did not show reactivity with viral proteins (data not shown).