Table 1.

Primers used for the mutagenesis of PGQM motif

Primer Designation	Sequence 5′ → 3′
Sph-UP	+	GGA	GCC	ACC	CCA	CAA	GAT	TT
Apa-DS	−	TCC	ACA	TTT	CCA	ACA	GCC	CT
P93	+	GGG	CCT	ATT	GCA	GCA	GGC	CAG	ATG	AGA
	−	TCT	CAT	CTG	GCC	TGG	TGC	AAT	AGG	CCC
G94	+	GGG	CCT	ATT	GCA	GCA	GGC	CAG	ATG	AGA
	−	TCT	CAT	CTG	GCC	TGC	TGC	AAT	AGG	CCC
Q95	+	ATT	GCA	CCA	GGC	GCG	ATG	AGA	GAA	CCA
	−	TGG	TTC	TCT	CAT	CGC	GCC	TGG	TGC	AAT
M96	+	GCA	CCA	GGC	CAG	GCG	AGA	GAA	CCA	AGG
	−	CTT	TGG	TTC	TCT	CGC	CTG	GCC	TGG	TGC
ΔPGQM	+	CTT	ATT	GCA	AGA	GAA	CCA	AGG	GGA	AGT
	−	TGG	TTC	TCT	TGC	AAT	AGG	CCC	TGC	ATG