Table 3.

Effect in alterations in PGQM motif on virus replication involving a single round

Proviral DNA	Titers, CFU/ml	Inhibition, %
ED84 (NL4-3)	1792	0
NL-P93A	768	42
NL-G94A	0	100
NL-Q95A	0	100
NL-M96A	1376	24
NL-ΔPGQM	0	100

No hygromycin-resistant colonies were observed for any of the proviral clones when the transcomplementation was performed without pSV-A-MLV-env, pSV-A-MLV-env by itself, or mock-transfected. Extent of inhibition was calculated in comparison to pED84 control proviral DNA. CFU, colony-forming units.