Figure 4.

SLM-1 associates with the p59^fyn and PLCγ-1 SH3 domains and the p59^fyn, PLCγ-1, GAP, and Grb2 SH2 domains. (A) myc-Sam68 and myc-SLM-1 were transfected in HeLa cells separately. The cell lysates were divided equally and incubated with affinity matrices containing GST alone, GST-fynSH3, GST-PLCγ-1SH3, or GST-GAPSH3. The bound proteins were identified by immunoblotting with anti-myc antibodies. The migration position of Sam68 and SLM-1 is shown. (B) myc-Sam68 and myc-SLM-1 were cotransfected with p59^fyn. The cell lysates were divided equally and incubated with affinity matrices containing GST alone, GST-fynSH2, GST-PLCγ-1SH2/2, GST-GAP2/3/2, or GST-Grb2SH2. The bound proteins were identified by immunoblotting with anti-myc antibodies.