SLM-1 and SLM-2 associate with Sam68. Vector alone, myc-SLM-1, myc-SLM-2, and myc-Sam68 were cotransfected with HA-Sam68 in HeLa cells as indicated. The transfected cells were lysed and the lysates were immunoprecipitated with IgG or anti-myc antibodies. The immunoprecipitated proteins (IP) were separated by SDS/PAGE and immunoblotted for the presence of HA-Sam68 with anti-HA antibodies. The migration positions of HA-Sam68 and the heavy chain of IgG are shown on the left. The expression of the myc-Sam68, -SLM-1, and -SLM-2 is shown to be equivalent (lanes 14–16).