Figure 2.

Serial deletions delineate the boundaries of the mPip ID. (A) Schematic representation of the C-terminal and internal deletion mutants used in this study. The solid box indicates the Pip ID. On the right, the ability of the different mutants to form TC. Equal amounts of proteins prepared by in vitro transcription/translation were assayed for interaction with PU.1 and λ1B probe. On the left (−), nonprogrammed reticulocyte lysate was used as control for nonspecific binding. (C) Pip and the deletion mutants ΔPip and Δ422–450 were tagged with a HA epitope to demonstrate the presence of Pip protein in the complex, in the presence of an anti-HA antibody.