Table 1.
kcat (min−1) and Km (μM) values for NTP-G6 and dNTP-G4 with GTP and dGTP
Species |
kcat
|
Km
|
Selectivity | ||
---|---|---|---|---|---|
dGTP | GTP | dGTP | GTP | ||
NTP-G6 | 2.74 × 10−5 | 1.46 × 10−3 | 918 | 425 | 115 |
dNTP-G4 | 4.43 × 10−4 | 1.90 × 10−5 | 98 | 106 | 26 |
Values were calculated with the Michaelis-Menten equation by using the data presented in Fig. 3, and each is the average of two replicate experiments. Selectivity is the ratio of the kcat/Km values with GTP and dGTP.