Table 1.
Affinities of finger-2 proteins
| Protein1 | Finger-2 helix2 | Finger-2 subsite3 | KD, nM4 | KD. Prot/KD. Zif268 |
|---|---|---|---|---|
| pGGG | SRSDHLTR | GGG | 0.4 | 0.04 |
| pmGGG | SRSDKLVR | GGG | 6 | 0.6 |
| GTG | >1,400 | |||
| pGGA | SQRAHLER | GGA | 3 | 0.3 |
| pmGGT | STSGHLVR | GGT | 15 | 1.5 |
| GGC | >2,400 | |||
| pmGGC | SDPGHLVR | GGC | 40 | 4.0 |
| pmGAG | SRSDNLVR | GAG | 1 | 0.1 |
| GGG | 45 | 4.5 | ||
| pmGAA | SQSSNLVR | GAA | 0.5 | 0.05 |
| pGAT | STSGNLVR | GAT | 3 | 0.3 |
| pmGAC | SDPGNLVR | GAC | 3 | 0.3 |
| GCC | 90 | 9.0 | ||
| pGTG | SRKDSLVR | GTG | 3 | 0.3 |
| pmGTG | SRSDELVR | GTG | 15 | 1.5 |
| GAG | 30 | 3.0 | ||
| pGTA | SQSSSLVR | GTA | 25 | 2.5 |
| GTG | >1,000 | |||
| pmGTT | STSGSLVR | GTT | 5 | 0.5 |
| pGTC | SDPGALVR | GTC | 40 | 4.0 |
| GCC | >4,400 | |||
| pmGCG | SRSDDLVR | GCG | 9 | 0.9 |
| GAG | 6 | 0.6 | ||
| pGCA | SQSGDLRR | GCA | 2 | 0.2 |
| GCT | 10 | 1 | ||
| pmGCT | STSGELVR | GCT | 65 | 6.5 |
| pGCC | SDCRDLAR | GCC | 80 | 8.0 |
| C7 | SRSDHLTT | TGG | 0.5 | 0.05 |
| Zif268 | SRSDHLTT | TGG | 10 | 1 |
1
Protein designations are as in Fig. 2.
2
Helix positions −1, 3, and 6 are shown in bold.
3
Altered nucleotides are underlined.
4
Values represent at least two independent experiments. The SE was ± 50%.