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. Author manuscript; available in PMC: 2006 Sep 29.
Published in final edited form as: Cell Microbiol. 2005 Jun;7(6):765–778. doi: 10.1111/j.1462-5822.2005.00509.x

Fig. 1.

Fig. 1

The autophagy enzymes Atg7 and Atg8 redistribute to phagosomes that contain virulent Legionella.

A. Macrophages infected for 5 min with post-exponential wild-type (PE WT) or avirulent dotA Legionella that express GFP were stained with Atg7-specific antibody. Arrows indicate position of bacteria. Scale bar = 10 μm.

B. The percentage Atg7-positive phagosomes was determined after infecting macrophages for the periods indicated with PE WT (white bars), exponential phase WT (black bars), PE dotA mutant (striped bars) or PE WT Legionella after pretreating macrophages with the autophagy inhibitor 3MA (grey bars). Values are the mean percentage colocalization ± SD calculated by scoring 100 bacteria per coverslip in at least three experiments.

C. Macrophages infected for 4 h with PE WT or dotA Legionella that express GFP were stained with Atg8-specific antibody. Arrows indicate position of bacteria. Scale bar = 10 μm.

D. The percentage Atg8-positive phagosomes was determined after infecting macrophages for the periods indicated with PE WT (white bars) or dotA mutant Legionella (grey bars). Values are the mean percentage colocalization ± SD calculated by scoring 100 bacteria per coverslip in two independent experiments.