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Phenotypes associate with the S53P form of TFIIB. The sua7–36-encoded S53P derivative confers Pho−, cold-sensitive, and heat-sensitive growth phenotypes. An isogenic strain pair expressing either normal TFIIB (WT) or the S53P derivative (S53P) was incubated on rich (YPD) medium at either 11°C, 30°C, or 37°C, or on phosphate-depleted medium (−Pi) at 30°C. Plates were photographed after 2 days of incubation, except for the 11°C plate, which was photographed after 14 days of incubation.
