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. 1999 Mar 16;96(6):2764–2769. doi: 10.1073/pnas.96.6.2764

Figure 5.

Figure 5

Pho4-mediated TFIIB conformational change. Susceptibility to V8 protease was used as a probe for TFIIB conformational change. The V8 protease digestion pattern of the wild-type (A) and S53P form (B) of TFIIB is shown. Either 0, 5, or 25 ng of V8 protease was added to each sample, either in the absence (lanes 1–3) or presence (lanes 5–8) of Pho4. Purified Pho4 was added at either 1 (lanes 5 and 7) or 4 μg (lanes 6 and 8); GST was added to bring the total protein concentration to 4 μg. ∗ in B denotes a Pho4-dependent proteolytic fragment unique to the S53P form of TFIIB.