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Effect of S53P on LexA-Adr1 activation of a lexA(op)-lacZ reporter. The lexA(op)-lacZ reporter plasmid was introduced into the isogenic strains YMH506 (SUA7+) and YMH507 (sua7–36) and assayed for β-galactosidase activities. Absolute activities are 1.6, 84, 1.1, and 19 units of β-galactosidase in columns 1–4, respectively. All assays were done in duplicate, using three independent transformants. Error bars denote SD and in all cases are less than 30%.
