Figure 5.

NT induces GSK-3α/β phosphorylation in SW480 and HCT116 cells. SW480 (A) or HCT116 (B) cells were serum-starved for 24 hours. Cells were treated with NT (100 nM) for 20 minutes. Total protein was extracted from cells and resolved by SDS-PAGE, transferred to PVDF membranes, and probed with anti-phospho-GSK-3α/β antibody; membranes were stripped and reprobed with anti-GSK-3α/β antibody. Signals from three separate experiments were quantitated densitometrically and expressed as fold changes with respect to total GSK-3α and PKCβ, respectively.