Skip to main content
. 1999 Mar 16;96(6):2800–2804. doi: 10.1073/pnas.96.6.2800

Figure 3.

Figure 3

Cell cycle-regulated association of xCdc7 with Mcm complexes. Interphase extracts or metaphase extracts were subjected to immunoprecipitation with xCdc7 antibodies (xCdc7 IP), xMcm3 antibodies (xMcm3 IP) or protein A alone (Control IP) followed by gel electrophoresis according to the method of Laemmli (ref. 38; see Materials and Methods) and immunoblotting with either xMcm3 antibodies or xCdc7 antibodies. The positions of xCdc7 and xMcm3 proteins are indicated by arrowheads on the right. xMcm3 coprecipitated with xCdc7 in interphase but not in metaphase. Control immunoblots show that the xCdc7-IPs from interphase and metaphase precipitated comparable amounts of xCdc7; and the xMcm3-IPs from interphase and metaphase precipitated comparable amounts of xMcm3 (data not shown).