Figure 5.

Assembly of focal adhesions and actin stress fibers is absent in unsupplemented FN−/− fibroblasts plated on the 120-kDa cell-binding domain of fibronectin (A). The cell spreading and assembly of focal adhesions and actin stress fibers as a result of supplementation with antibodies directed against the ectodomain of mouse syndecan-4 (B) is inhibited by the C3 exotransferase treatment that inhibits Rho (C). Supplementation with LPA leads to full spreading and assembly of focal adhesions and actin stress fibers in cells plated on the 120-kDa cell-binding domain of fibronectin alone (D).