Table 1.
Summary of morphology of FN−/− fibroblasts cultured under various conditons
| Substrate | Supplement | % of attached cells that assemble focal adhesions and actin stress fibers | Corresponding figures |
|---|---|---|---|
| FN | None | 98 | 4A |
| 120 kDA | None | 6 | 4B |
| 40 kDa | 47 | 4C | |
| Anti-syndecan-4 antibodies | 90 | 4D | |
| IgG | 5 | 4E | |
| Anti-β1 mAb | None | 4 | |
| Anti-syndecan-4 antibodies | 85 | 4F | |
| IgG | 6 | 4G | |
| Polylysine | None | 5 | |
| Anti-syndecan-4 antibodies | 6 | 4H | |
| IgG | 5 | 4I | |
| 120 kDa | None | 6 | 5A |
| Anti-syndecan-4 antibodies | 89 | 5B | |
| Anti-syndecan-4 antibodies + C3 | 8 | 5C | |
| LPA | 95 | 5D |
A minimum of 50 cells were counted for each experiment. FN, fibronectin; 120 kDa, 120 kDa α-chymotryptic cell-binding domain of fibronectin; anti-β1 mAb, rat monoclonal antibody directed against mouse β1 integrin chain; 40 kDa, 40 kDa α-chymotryptic heparin-binding domain of fibronectin; anti-syndecan-4 antibodies, the antibodies are directed against the ectodomain of mouse syndecan-4 core protein; C3, C3 exotransferase; LPA, lysophosphatidic acid.