Table 2.

The antiproliferative effect of angiostatin is reversed by anti-α subunit ATP synthase antibody

Concentration angiostatin added, μM	Percent proliferation inhibited, ±SEM
	Without antibody	With antibody	% Recovery
0	0	0	0
0.5	10  ±  1.4	1  ±  0.2	90
0.75	25  ±  4.2	5  ±  4.1	80
1.0	23  ±  9.0	6  ±  0.8	74

HUVEC were plated at a density of 5,000 cells/well in media containing angiostatin at a final concentration of 0.5, 0.75, and 1.0 μM. Anti-α subunit ATP synthase antibody derived from E. coli was added concomitantly at a dilution of 1:10. MTS/PMS solution was added and absorbance of formazan was quantitated according to the manufacturer’s specifications. Results represent three separate experiments performed in duplicate with SEM. Percent recovery represents the ability of the anti-α-subunit ATP synthase antibody to block the antiproliferative effect of angiostatin, and thereby restore proliferation to an average of 81% of that obtained with the control cells.