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An in vitro centriole separation assay. Deconvolution images of centriole doublets and singlets. (A and A′) These doublets represent the 0-h starting point of the assay. (B and B′) At the 1-h endpoint, the majority of centrosomes are singlets, as depicted. (A and B) α-Tubulin staining. (A′ and B′) γ-Tubulin staining. (Bar = 1 μm.)
