Figure 3.

The dependence of centriole separation on cyclin-dependent kinases. The graphs express centriole separation activity as conversion percentages, as described in Table 2. (A) Depletion and rescue of centriole separation activity with Suc1p and cyclin E/cdk2. (B) Depletion of both cyclin E- and cyclin A-dependent kinases inhibits centriole separation. The conversion percentage has been normalized to the control depletion (uncoupled protein A-Sepharose). (C) The mitotic state inhibits centriole separation.