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Normal polarization of the actin cytoskeleton in a strain carrying 6-histidine-tagged ACT1 gene. (A) Control PR/pCENWT strain, carrying the ACT1 gene for wild-type actin. (B) Strain PR/pRSWT6his, carrying the ACT1 gene expressing 6-histidine-tagged actin. Rhodamine-phalloidin staining. (Bar = 5 μm.)
