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. 1999 Mar 16;96(6):2828–2833. doi: 10.1073/pnas.96.6.2828

Figure 4.

Figure 4

Localization of GFP-fusion proteins of Ubp-M in cells transfected with GFP-Ubp-M fusion genes. (A) Constructs of GFP-fusion genes with different domains of Ubp-M that were transfected into cultured cells. DNA fragments containing the catalytic domain contained codons for either cysteine or serine at position 205. (B) Enzymatically active GFP-Ubp-M localizes to the cytoplasm of cells fixed either 18 hr or 72 hr after transfection. The nuclei of the same cells are stained with DAPI. (C) Inactive mutant GFP-Ubp-M (C-S) also localizes to the cytoplasm of cells fixed 18 hr after transfection, but, by 72 hr, most of the GFP staining is nuclear. The nuclei of the same cells also are stained by DAPI. Cells were processed for fluorescence microscopy as described.