Figure 5.

Immunofluorescence colocalization of rab11 and membrane-associated rab11BP in endosomes and the pericentriolar recycling compartment. Permanent transformants of Chinese hamster ovary cells that express the human transferrin receptor (TRVb-1 cells) were transfected with a plasmid-encoding HA-tagged rab11BP. The cells grown on coverslips were permeabilized with 0.05% saponin in PBS for 4 min to release soluble cytosolic components and processed for double-label immunofluorescence with primary mouse mAbs to the HA epitope (A) and rabbit antibodies to rab11 (B), followed by Texas red-conjugated donkey anti-mouse antibody and fluorescein-conjugated donkey anti-rabbit antibody, respectively. The arrows and arrowheads point to the pericentriolar compartment and to peripheral-sorting endosomes in a transfected cell, respectively. Note that a trio of untransfected cells in B shows intense staining of the pericentriolar compartment with anti-rab11 antibodies.