Figure 7.

Inhibition of transferrin recycling by truncated rab11BP(1–504) and its relief by the nonprenylated rab11ΔC. Rates of transferrin recycling were assessed as described previously (16) in cultures of TRVb cells, a mutant line of Chinese hamster ovary cells lacking a functional endogenous transferrin receptor, that were cotransfected with a plasmid encoding the human transferrin receptor (A–D) and plasmids encoding the intact rab11BP (A), or its truncated variants, rab11BP (1–336) (B) and rab11BP (1–504) (C and D). A also shows the recycling kinetics (▵) for cells that express the receptor together with the rab11 dominant negative mutant (rab11S25N). In D, the cells express—in addition to the transferrin receptor and the truncated rab11BP(1–504)—the nonprenylatable rab11ΔC. In A–D, the recycling kinetics also are shown (○) for control cells that were cotransfected with the plasmid encoding the transferrin receptor and the pCDNA3 vector lacking an insert.