Figure 6.
STAT3 hemizygous cells are impaired for undifferentiated clonal growth. (A) Efficiency of clonal stem cell growth of wild-type E14 cells (filled bars) or Stat3+/− hemizygous cells (empty bars). The proportion of alkaline phosphatase-positive (undifferentiated) colonies was measured after 6 days of growth at the indicated concentrations of LIF (units/ml). Data are average of duplicate assays. (B) Dose-response effect of LIF on Stat3+/flox (filled bars) or Stat3+/− (empty bars) cell proliferation. [3H]Thymidine incorporation (cpm, mean and SD of triplicate samples) was measured on 4-day cultures.