Table 1.
Comparison of Housekeeping Gene Expression in Various Cell Linesa
| Cell line | Housekeeping Genes | |||
| ATP1A1 b | GAPDH | HPRT1 | PMCA4 | |
| Human Salivary Gland cells | 19.62 ± 0.38 | 16.96 ± 0.11 | 21.33 ± 0.21 | 20.61 ± 0.14 |
| HCT-116 (colorectal carcinoma) | 20.4 ± 0.67 | 17.33 ± 0.4 | 21.71 ± 0.31 | 21.7 ± 0.36 |
| KB-3-1 (Cervical squamous cell carcinoma) | 20.57 ± 0.36 | 17.73 ± 0.07 | 20.49 ± 0.57 | 22.13 ± 0.1 |
| SNB-75 (Glioblastoma) | 19.77 ± 0.27 | 17.49 ± 0.15 | 21.9 ± 0.2 | 19.77 ± 0.17 |
| SF-295 (Glioblastoma) | 20.62 ± 0.34 | 17.01 ± 0.43 | 21.48 ± 0.28 | 21.61 ± 0.13 |
| MCF-7 (breast adenocarcinoma) | 18.49 ± 0.28 | 17.1 ± 0.33 | 20.69 ± 0.13 | 21.39 ± 0.24 |
| UACC-62 (melanoma) | 18.66 ± 0.8 | 16.31 ± 0.5 | 21.22 ± 0.25 | 20.58 ± 0.3 |
| COR-L23P (lung carcinoma) | 18.14 ± 0.34 | 18.12 ± 0.24 | 20.54 ± 0.21 | 21.03 ± 0.17 |
aThe LightCycler RNA Master SYBR Green Kit and LightCycler 480 were used in these RT-PCR studies. The average crossing point values are given for the different cell lines ± SD (n = 4 for all samples).
bF-test comparing all values for PMCA4 and ATP1A1 indicates that there is a statistically significant difference (p = 0.041). No statistically significant difference is seen between PMCA4 and GAPDH (p = 0.093) or PMCA4 and HPRT1 (p = 0.074)