Table I.
Segregation of the S-RNase alleles in the progenies of controlled field crosses and self-pollinations
S-genotypes were determined by PCR. Observed S-RNase genotypes, expected segregation ratios, and χ2 values obtained for each population are indicated.
| Seed Parent (S-Genotype) | Pollen Parent (S-Genotype) |
S-Genotypes Observed
|
Total | Expected Segregation Ratio | χ2d (P Value) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| S1SC | S2SC | S1S2 | S2S2 | S1S1 | SCSC | |||||
| Goldrich (S1S2) | Currot (SCSC) | 31 | 39 | – | – | – | – | 70 | 1:1 | 0.91 (0.339) |
| Goldrich (S1S2) | Canino (S2SC) | 66 | 55 | 28 | 22 | – | – | 171 | 2:2:1:1b | 2.98 (0.394) |
| Canino (S2SC) | Canino (S2SC) | – | 53 | – | 11 | – | 35 | 99 | 3:1:2b | 2.20 (0.333) |
| GC-8 (S2SC)a | GC-8 (S2SC) | – | 14 | – | 4 | – | 6 | 24 | 3:1:2b | 0.83 (0.659) |
| GC-10 (S2SC)a | GC-10 (S2SC) | – | 14 | – | – | – | 10 | 24 | 1:1c | 0.67 (0.414) |
| GC-80 (S1SC)a | GC-80 (S1SC) | 15 | – | – | – | 3 | 6 | 24 | 3:1:2b | 1.50 (0.472) |
| GC-86 (S1SC)a | GC-86 (S1SC) | 13 | – | – | – | – | 11 | 24 | 1:1c | 0.17 (0.683) |
a
Seedlings derived from the cross Goldrich (S1S2) × Canino (S2SC).
b
Expected ratios for a single mutation unlinked to the S-locus.
c
Expected ratios for nonmutated GC seedlings.
d
Observed ratios do not differ significantly from expected at P < 0.05 in any case.