Figure 1.

Molecular analysis of representative pschit33 transgenic plants. A, Northern analysis of tobacco pschit33 transgenic lines (33.4, 33.5, 33.6, 33.9, 33.10) and control line (Bin19) grown in standard conditions. As a positive control, total RNA of T. harzianum (T.h.) grown in chit33-inducing conditions was used (Dana et al., 2001). B, Western analysis of total proteins from leaf extracts of control and transgenic tobacco lines. As a positive control, culture supernatant of T.h. grown in chit33-inducing conditions was used. C, Specific chitinase activity in protein-soluble extracts of control and transgenic tobacco lines. 1 U = 1 nmol of 4-methylumbelliferone released per minute. D, Bin19 and pschit33 plants grown for 5 weeks.