Figure 5.
Abiotic stress tolerance of fungal chitinase-overexpressing plants. A, Salt stress tolerance. Seven-day-old seedlings grown in one-half-strength Murashige and Skoog basal salt medium were transferred to agar-water plates containing 256 mm NaCl. The effects of salt on plant growth and viability after 7 d at 25°C and continuous light allow the division of plant populations of each of the transgenic lines tested into three categories: unaffected plants (no visible effects, sustained growth, and viability), partially affected plants (deleterious effects, marked by growth arrest, and chlorosis symptoms), and dead plants. B and C, Cd and Cu tolerance. The assays were made as in A, substituting NaCl for 300 μm CdSO4 (B) and 300 μm CuSO4 (C) in the selective plates.