Figure 5.

The tnp mutant contains a deletion in the promoter of LEC1, resulting in ectopic expression of LEC1. A, Mapping of the TNP locus. BAC clones and the number of recombinants are shown. B, Semiquantitative RT-PCR analysis of LEC1 and LEC2 transcript levels using RNA extracted from tnp and pls seedlings 1 to 2 d postgermination. Arrow indicates the size of the LEC2 specific product. ACT1 is the amplification control. Control experiments lacking reverse transcriptase showed no amplification products (data not shown). C, Schematic diagram indicating the position of the 3,256 bp deletion of the LEC1 promoter in tnp mutants relative to the transcriptional start site of the LEC1 gene.