Table I.
The effect of the methylation inhibitor 5-azacytidine on penetrance of the tnp mutant phenotype
Three independent T3 sibling lines (1, 7, and 21) were grown on 1/2× MS 10 medium or medium supplemented with 100 μm 5-azacytidine (5-AZA-C). The number of seedlings with a wild-type appearance (TNP) or showing a tnp mutant phenotype (tnp) was counted 7 d postgermination.
| T3 Line | Medium | TNP | tnp | % tnp |
|---|---|---|---|---|
| 1 | 1/2× MS 10 | 53 | 21 | 28 |
| 1 | 100 μm 5-AZA-C | 30 | 49 | 62 |
| 7 | 1/2× MS 10 | 120 | 0 | 0 |
| 7 | 100 μm 5-AZA-C | 112 | 9 | 7 |
| 21 | 1/2× MS 10 | 75 | 52 | 41 |
| 21 | 100 μm 5-AZA-C | 71 | 65 | 48 |