Figure 7.

Functional complementation of an E. coli xylB deletion mutant by At-XK isoforms. DY329-xylB∷cat E. coli cells were transformed with pET (XK⁻), pET-Ec-XK (XK⁻:XK), pET-At-XK1 (XK⁻:A1), or pET-At-XK2 (XK⁻:A2), and, along with a nontransformed DY329 strain (WT), plated on LB, LB supplemented with chloramphenicol (LB-CHL), minimum M9 supplemented with 0.05% yeast extract and with 0.2% Glc (Glc), or minimum M9 medium supplemented with 0.05% yeast extract and 0.2% Xyl (Xyl) medium. All plates were supplemented with 1 mm IPTG and were incubated at 30°C until colonies appeared.