Figure 9.

Genetic characterization of Arabidopsis knockout mutants in At-xk1 and At-xk2 alleles A, A scheme of the At-xk1∷T-DNA and At-xk2∷T-DNA loci. The gene structure shows exons (black rectangles), and 5′- and 3′-untranslated regions (white triangles). The pROK2 T-DNA is represented below, and primers used for genomic-PCR and RT-PCR analysis are indicated by arrowheads. B, Characterization of T-DNA insertion locus by PCR using the primer sets as indicated and DNA template isolated from wild type (Col-0; WT) and from mutants At-xk1∷T-DNA (A1) and At-xk2∷T-DNA (A2). C, Semiquantitative RT-PCR analysis of the actin 2 (At3g18780, control), Atxk-1, and Atxk-2 mRNAs in wild-type and mutant plants as described in “Materials and Methods.” The black bars in A represent the part of the mRNA analyzed by RT-PCR using the primer sets RTAtXK1F/RTAtXK1R and RTAtXK2F/RTAtXK2R.