Table II.
Pro hydroxylation and monosaccharide composition of the SynGMs expressed in Arabidopsis
tr., Percentages lower than 1% are given as trace.
| SynGM | Pro Hydroxylationa | Monosaccharide Composition of the O-Glycans Linked to the SynGMsb
|
||||
|---|---|---|---|---|---|---|
| Gal | Ara | Rha | GlcA | Gal:Ara (Molar Ratio) | ||
| % | mol % | |||||
| AGPv motif | ||||||
| (VP)11 | 59.0 (approximately 7 Hyp) | 34.7 | 38.3 | 26.9 | n.d.c | 1:1.10 |
| (VP)11 (Tob)d | 79.5 (approximately 9 Hyp) | 40 (46) | 39.0 (44.8) | 8.0 (9.2) | 13 | 1:0.97 |
| (SP)32 | 95.3 (approximately 31 Hyp) | 57.7 | 30.1 | 12.2 | n.d. | 1:0.52 |
| (SP)32 (Tob)e | 100 (approximately 32 Hyp) | 45 (55.0) | 28 (34.5) | 8.0 (9.9) | 19 | 1:0.62 |
| Extensin motif | ||||||
| (SPPP)15 | 71.0 (approximately 35 Hyp) | +++g | ++ | ± | n.d. | n.d. |
| (SPPP)15 (Tob)f | 73.3 (approximately 36 Hyp) | 37 (41.0) | 42 (46.6) | 7.0 (7.7) | 10 | 1:1.13 |
| (SPPPP)18 | 51.3 (approximately 37 Hyp) | +++ | ++ | ± | n.d. | n.d. |
| (SPPPP)18 (Tob)h | 97.5 (approximately 72 Hyp) | 5 | 95 | – | tr. | 1:19.0 |
| (YK)20 | 61.1 (approximately 110 Hyp) | 57.2 | 29.0 | 13.8 | n.d. | 1:0.51 |
| (YK)20 (Tob)i | 100 (approximately 180 Hyp) | 2.8 | 90 | 1 | – | 1:11.2 |
| AGP | ||||||
| LeAGP1 | 94.7 (approximately 44 Hyp) | 68.9 | 18.4 | 12.7 | n.d. | 1:0.27 |
| LeAGP1 (Tob)jk | 92.3 (approximately 43 Hyp) | 37 (42) | 39 (44.3) | 8 (9.0) | 12 | 1:1.05 |
a
Based on the amino acid composition of the purified SynGM-EGFP fusions. In parentheses, the total number of hydroxyproline units per construct is shown.
b
Monosaccharide composition before and after discounting the uronic acids (GlcA) for comparison.
c
n.d., Not determined.
d
From Tan et al. (2003).
e
From Shpak et al. (1999).
f
From Shpak et al. (2001).
g
+++, Abundant; ++, intermediate; ±, scarce.
i
From Held et al. (2004).
j
From Zhao et al. (2002).
k
Four percent of mannose was also detected.