Figure 6.
Phylogenetic analysis of various plant PEPC isoforms (based on the last 70 amino acid residues of the 3′ side of the coding region) using a neighbor-joining consensus tree including the single-cell functioning C4 species B. sinuspersici and S. aralocaspica. The tree was oriented with PEPC amino acid sequence from Escherichia coli and Arabidopsis bacterial-type PEPC as functional outgroups. The gray box indicates the distribution of members of the Chenopodiaceae family whose sequences have been obtained in this work. The GenBank accession numbers of the sequences used to construct the phylogenetic tree are shown in the figure. Bootstrap analysis was carried out with 1,000 replicates. Bootstrap values of the branches are indicated in a 100 basis.