Figure 3.

Overexpression of C1 and coregulation of C3. A, The overexpression construct used for C1 is shown. Promoter indicates the truncated hsp70A/rbcS2 tandem promoter along with the first intron of rbcS2 that bears an enhancer (see “Materials and Methods”). The tagged c1 cDNA bears the major part of the c1 ORF (the first 23 amino acids are missing) and the entire 3′ UTR (see Supplemental Protocol S1). B and C, Different amounts of proteins from a crude extract (90, 60, and 30 μg per lanes) of wild-type (WT) cells were separated on SDS-PAGE and used for western analysis with anti-C1 (B) and anti-C3 (C) antibodies along with proteins of crude extracts (30 μg per lane) from two different C1-overexpressing strains (C1-ox₁₈ and C3-ox₉₃). The positions of C1 and C3 are indicated by arrowheads.