Skip to main content
. 2006 Oct;142(2):642–650. doi: 10.1104/pp.106.085878

Figure 5.

Figure 5.

Complementation of the sig6-2 knockout mutant. A, T-DNA and AtSig6 detection by Southern-blot hybridization of total genomic DNA from wild-type, knockout, and complementation line plants. Left, Primary T-DNA insertion. DNA digested with EcoRV and hybridized with a sulf probe (see Fig. 1). Middle, Secondary T-DNA insertion after retransformation of sig6-2 knockout line. DNA digested with HindIII and hybridized with nptII probe. Right, AtSig6 detection. DNA digested with EcoRI and hybridized with Sig6-specific probe. Fragment sizes (kb) are given in the left margin of each image. B, Transcript analysis using total RNA from wild type (WT), sig6-2 (MT), and the sig6-2 complementation line (C). Top left, RT-PCR products with AtSig6-specific primers. Left and right, RNA gel-blot hybridization. The probes and transcript sizes (kb) are indicated in the left margins and ethidium bromide-stained 25S rRNA is shown below each image.