Table I.
Estimated off-target patterns during PTGS in plants
| Species | Source Sequence Set | Release Date | Total No. Unique Gene Transcripts | No. Triggers Predicted to Silence Off-Targets (% Total) | Average No. Predicted Off-Targets/Trigger | Average No. Predicted Off-Target Regions/Off-Target | Average Length (nt)/Off-Target Region | Average No. Efficient siRNAs/Trigger |
|---|---|---|---|---|---|---|---|---|
| Arabidopsis | ATH1 | 6/10/04 | 28,952 | 19,882 (68.7) | 3.9 | 2.9 | 41.2 | 35.2 |
| Arabidopsis | AGI 11 | 1/29/04 | 45,683 | 37,250 (81.5) | 4.3 | 3 | 99.2 | 147.5 |
| Capsicum annuum | CaGI 1 | 6/4/04 | 10,712 | 27,88 (26.0) | 1.9 | 2.2 | 40.7 | 12.5 |
| Gossypium spp. | CGI 5 | 9/16/03 | 24,350 | 14,270 (58.6) | 6.9 | 2.3 | 74 | 87.5 |
| Chlamydomonas reinhardtii | ChrGI 4 | 1/31/04 | 30,339 | 16,579 (54.6) | 6.1 | 1.9 | 40.2 | 12.5 |
| Glycine max | GmGI 11 | 9/16/03 | 67,826 | 49,658 (73.2) | 9.4 | 2.1 | 77.9 | 108.4 |
| Helianthus annuus | HaGI 3 | 9/16/03 | 20,520 | 11,961 (58.3) | 15.8 | 1.4 | 34.6 | 38.8 |
| Hordeum vulgare | HvGI 8 | 1/30/04 | 49,190 | 32,297 (65.6) | 16.3 | 2.6 | 99.4 | 205.9 |
| Tomato | LeGI 9 | 5/19/03 | 31,012 | 15,340 (49.5) | 2.4 | 2.3 | 60.8 | 30.9 |
| Lotus japonicus | LjGI 2 | 5/16/03 | 11,025 | 2,951 (26.8) | 2.6 | 1.7 | 35.6 | 10 |
| Lettuce | LsGI 2 | 2/3/04 | 22,185 | 12,345 (55.6) | 24.1 | 1.4 | 30.4 | 26.8 |
| Mesembryanthemum crystallinum | McGI 4 | 5/15/03 | 8,455 | 3,459 (42) | 13.3 | 2 | 33 | 29.4 |
| Medicago truncatula | MtGI 7 | 5/19/03 | 36,976 | 19,468 (52.7) | 5.6 | 2.2 | 49.4 | 50.6 |
| Nicotiana benthamiana | NbGI 1 | 1/28/04 | 6,118 | 31,99 (52.3) | 2.8 | 3.8 | 53.4 | 42.1 |
| Nicotiana tabacum | NtGI 1 | 6/4/04 | 10,232 | 2,184 (21.3) | 1.8 | 3.1 | 38.3 | 15.2 |
| Allium cepa | OnGI 1 | 9/17/03 | 11,726 | 3,735 (31.9) | 1.7 | 2.3 | 54.2 | 20.2 |
| Rice | OsGI 15 | 5/27/04 | 88,765 | 45,406 (51.2) | 22.5 | 4.4 | 41.7 | 174.3 |
| Pinus spp. | PGI 4 | 1/28/04 | 31,771 | 16,487 (51.9) | 11.7 | 5.5 | 38.4 | 27.5 |
| Rye | RyeGI 3 | 1/29/04 | 5,347 | 1,472 (27.5) | 2.7 | 6 | 32.4 | 10.7 |
| Sorghum bicolor | SbGI 8 | 5/26/04 | 39,148 | 22,039 (56.3) | 6.4 | 1.9 | 70.3 | 56.9 |
| Sugarcane | SoGI 1 | 1/30/04 | 95,884 | 55,269 (57.6) | 24.1 | 3.5 | 69.1 | 205.4 |
| Solanum tuberosum | StGI 9 | 5/26/04 | 32,553 | 20,469 (62.9) | 4.4 | 2.9 | 46.2 | 42.2 |
| Triticum aestivum | TaGI 8 | 1/29/04 | 12,3807 | 89,862 (72.6) | 19.5 | 2.7 | 52.4 | 102.2 |
| Theobroma cacao | TcaGI 1 | 6/4/04 | 2,539 | 531 (20.9) | 2.6 | 1.9 | 37.4 | 13 |
| Vitis vinifera | VvGI 3 | 11/14/03 | 23,109 | 10,548 (45.6) | 8.8 | 1.8 | 40.3 | 37.1 |
| Zea mays | ZmGI 14 | 1/2/04 | 56,364 | 40,028 (71) | 8.9 | 3 | 63.1 | 96.9 |