Table II.
Result from the siRNA Scan search with Arabidopsis BTI1 coding sequence as a trigger
TC numbers in bold are the ones used for quantitative RT-PCR.
| Off-Target ID | Identical (I) or Reverse Complementary (RC) Sequence | BLAST Search |
|---|---|---|
| NP167859 | I, AGCCTGTTCATAAGGTTCTCGG | Arabidopsis reticulon family protein (RTNLB6) (At3g61560). |
| NP302761 | I, AGAAGAAGAAGACTAAGAAGCI, AGCCTGTTCATAAGGTTCTCGG | Contains similarity to DnaJ gene YM8520.10 gb|825566 from Saccharomyces cerevisiae cosmid gb|Z49705. ESTs gb|Z47720 and gb|Z29879 come from this gene. |
| TC251496 | RC, TGATTCTTCTTCGTCTTCTTC | GB|AAM51226 Unknown protein {Arabidopsis}; similar to UP|Q6Z702 (Q6Z702) putative 3-isopropylmalate dehydratase large subunit. |
| TC251703 | I, CCTGTTCATAAGGTTCTCGGCG; I, TGGTCTAATGCCACTATGTTCATT;I, CCAAAGATTCCTGAAGTTCATATCCCTGAAGAACCT;I, TTCTTGACATTGGCATACATAGCTCT | GB|AAP47461.1|32331867|AY164887 RTNLB2 {Arabidopsis}, complete. Recently identified as BTI2. |
| TC255665 | I, TCTTCGTCTTCTTCATCTTCT | GB|AAD31078.1|4850408|F3F19 Contains PF|00097 zinc finger (C3HC4) ring finger motif. {Arabidopsis}, complete. |
| TC256637 | RC, TTCTTCTTCGTCTTCTTCATC | PIR|B96716 Probable Ser/Thr kinase F23O10.20 [imported]—Arabidopsis. |
| TC258543 | I, AGCCTGTTCATAAGGTTCTCGG | GB|AAP47457.1|32331859|AY164883 RTNLB6 {Arabidopsis}, partial (90%). |
| TC262843 | I, CTTCTTCGTCTTCTTCATCTTCT | UP|O49467 (O49467) Hypothetical protein F24J7.50 (hypothetical protein AT4g19490). |
| TC263798 | RC, TTCTTCTTCGTCTTCTTCATC | GB|AAO50470 Unknown protein {Arabidopsis}. |
| TC265975 | I, AGAAGAAGAAGACTAAGAAGC | UP|080799 (080799) T8F5.5 protein; weakly similar to PIR|S64314 probable membrane protein YGR023w—yeast (S. cerevisiae). |
| TC269146 | I, CTTCTTCGTCTTCTTCATCTTCT | UP|O49467 (O49467) Hypothetical protein F24J7.50 (hypothetical protein AT4g19490). |
| TC275407 | RC, TTCTTCTTCGTCTTCTTCATC | UP|Q8VYC1 (Q8VYC1) Putative Ser/Thr kinase. |
| TC275528 | I, GAGAAGAAGAAGACTAAGAAG | UP|Q9FJR9 (Q9FJR9) Similarity to maturase-related protein, complete. |
| TC275625 | RC, TTCTTCTTCGTCTTCTTCATCTTCT | GB|AAM51439 Unknown protein {Arabidopsis}; weakly similar to UP|081812(081812) auxilin-like protein, partial (13%). |