FIG. 9.

Proteins of the Sp family bind to region 10 of the rat SF-1 promoter. EMSAs were performed with a radiolabeled probe spanning regions 9 and 10 (9/10) and nuclear extracts from either Sertoli or MA-10 cells. Where indicated above the lanes, experiments were carried out in the presence of 150-fold molar excess of competitor oligodeoxynucleotides or antibodies to the transcription factors Sp1, Sp2, Sp3, Wt1, and Egr1. Sequences of probe and competitors are given in Table 2. Arrows indicate the positions of the major specific complexes, whereas asterisks indicate the supershifted complexes.