TABLE 1.
Structures of mutagenic oligonucleotides.
| Mutanta | Oligonucleotideb |
|---|---|
| μ7 | GGCCTCCGGTTCAAAAAAACCACCCATG (F) |
| μ8 | GGGCCTCCGGTGGCCCCCCCAAGAAACCGAGGGGA (F) |
| μ9 | GGGCCTCCGGTGGCCCCCCCCCACCCATTCTTTTAGGAGG (F) |
| μ10 | GGGCCTCCGGTGGCCCCCCCCCACCCATGAGGGGCTTC (F) |
| μ11 | GGGCCTCCGGTGGCCCCCCCCCACCCATGAGGGGAGGA (F) |
| μ10.1 | GGGCCTCCGGTGGCCCCCCCCACCCATGAGGGGAGGCGGAGAGGACGATCGGACAGG (F) |
| μ12 | GGGGGAAGCTTCAGCGGGCAGGCAGCGGCGGACTGC AAACTGGTTTGAGCCGATCGTCC (R) |
| μ13 | BBBBBAAGCTTCAGCGGGCAGGCAG CGGCGGACTGCCCCGGCCCCCTGTCCGATCGTCC (R) |
| μ14 | GGGGGAAGCTTCAGC GGGCAGGCAGATTATTTTTTTAAACTGGCCCTGTCCG (R) |
| μ15 | GGGGGAAGCTT CAGCGGGCCAAGTTCGGCGGACTGCAAACTGG (R) |
| μ16 | GGGGGAAGCTTGCTTT TTGAGGCAGCGGCGGACTGC (R) |
| μ1.1 | AAAGGCCTGCAGAGTCAGCTGGGGGCAGAGACC (F) |
| μ1.2 | AAAGGCCTGCAGATACACGTGTAGGCAGAGACC (F) |
| μ1.3 | AAAGGCCTGCAGAGTCACATGGGGGCAGAGACC (F) |
| μ1.4 | AAAGGCCTGCAGAGTAACGTGGGGGCAGAGACC (F) |
| μ1.5 | AAAGGCCTGCAGAGTCAGATGGGGGCAGAGACC (F) |
| μ3.1 | AAGGCCTGCAGAGTCACGTGGGGGCTCTGACCAATTGGGCCTCCGGTGGCCCCCC (F) |
| μ3.2 | AAGGCCTGCAGAGTCACGTGGGGGCAGACTCCAATTGGGCCTCCGGTGGCCCCCC (F) |
| μ4.1 | AAGGCCTGCAGAGTCACGTGGGGGCAGAGAGGAATTGGGCCTCCGGTGGCCCCC (F) |
| μ4.2 | AAGGCCTGCAGAGTCACGTGGGGGCAGAGACCAAATGGGCCTCCGGTGGCCCCCC (F) |
| μC | AAGGCCTGCAGAGTCACGTGGGGGCTTCTTTTCCGTGGGCCTCCGGTGGC (F) |
| Ins3 | AAGGCCTGCAGAGTCACGTGGGAGAGGCAGAGACCAATTGGGC (F) |
| Ins5 | AAGGCCTGCAGAGTCACGTGGGAGAGAGGCAGAGACCAATTGGGC (F) |
| Ins7 | AAGGCCTGCAGAGTCACGTGGGAGAGAGAGGCAGAGACCAATTGGGC (F) |
| Ins10 | AAGGCCTGCAGAGTCACGTGGGAGAGAGACCTGGCAGAGACCAATTGGGC (F) |
| Ins15 | AAGGCCTGCAGAGTCACGTGGGAGAGAGACCTAGAGAGGCAGAGACCAATTGGGC (F) |
| Ins20 | AAGGCCTGCAGAGTCACGTGGGAGAGAGACCTAGAGAGACCTGGCAGAGACCAATTGGGC (F) |
| 3′Ins20 | AAGGCCTGCAGAGTCACGTGGGGGCAGAGACCAATAGAGAGACCTAGAGAGACCTTGGGCCTCCGGTGGCC (F) |
| μ1/μC | AAGGCCTGCAGAGTAGTTCTGGGGCTTCTTTTCCGT (F) |
| +49 | GGGGAAGCTTCAGGAACTTCTTCCCCCAGC (F) |
| +30 | GGGGAAGCTTCAGC GGGCAGGAGCGGC (F) |
a
Sequences of complementary oligonucleotides and specifics of mutant generation given in Materials and Methods.
b
All PCR oligonucleotides presented 5′ to 3′ together with their respective orientations. F, Forward; R, reverse.