TABLE 2.
Sequences of oligodeoxynucleotides used for EMSA.
| Oligodeoxynucleotide (position)a | Sequenceb |
|---|---|
| E/C (−90 to −55) | TGCAGAGT CACGTG GGGGC AGAGACCAAT TGGGCCT |
| μE/C (−90 to −55) | TGCAGAGT agttct GGGGC AGAGACCAAT TGGGCCT |
| E/μ2/C (−90 to −55) | TGCAGAGT CACGTG acttt AGAGACCAAT TGGGCCT |
| E/μC (−90 to −55) | TGCAGAGT CACGTG GGGGC ttcttttccg TGGGCCT |
| E box (−94 to −65) | GGCC TGCAGAGT CACGTG GGGGC AGAGACC |
| μ1.1/C (−90 to −55) | TGCAGAGTCAgcTGGGGGCAGAGACCAATTGGGCCT |
| μ1.3/C (−90 to −55) | TGCAGAGTCACaTGGGGGCAGAGACCAATTGGGCCT |
| μ4.1 (−90 to −55) | TGCAGAGT CACGTG GGGGC AGAGAggAAT TGGGCCT |
| μ4.2 (−90 to −55) | TGCAGAGT CACGTG GGGGC AGAGACCAAa TGGGCCT |
| CCAAT box (−73 to −54) | GC AGAGACCAAT TGGGCCTC |
| C/EBP | TGCAGATTGCGCAATCTGCA |
| NF-Y | GAGACCGTACGTGATTGGTTAATCTCTT |
| Sp1 | ATTCGATCGGGGCGGGGCGAGC |
| Egr1 | GGGAGTCAGTCTTGCGTGGGCGTTAGTCAGTCGGG |
| NS | CTAGAGTCGACCTGCAGGCATGCAAGCTTGGCATTC |
| 9/10 (−41 to −6) | CCACCCATGAGGGGAGGAGGAGAGGACGATCGGACA |
| μ9/10 (−41 to −6) | CCACCCATtcttttAGGAGGAGAGGACGATCGGACA |
| 9/μ10 (−41 to −6) | CCACCATGAGGGGcttcaacttGGACCATCGGACA |
| 14 (−5 to +25) | GGGCCAGTTTGCAGTCCGCCGCTGCCTGCC |
| μ14 (−5 to +25) | GGGCCAGTTTaaaaaaataatCTGCCTGCC |
| 16 (+8 to +40) | AGTCCGCCGCTGCCTGCCCGCTGCTGGGGGAAGTTGTT |
| μ16 (+8 to +40) | AGTCCGCCGCTGCCTcaaaaagcCTGGGGGAAGTTGTT |
a
Positions given relative to the major transcriptional start site at +1, based on the rat SF-1 promoter sequence (see Fig. 1A).
b
EMSA oligonucleotides shown as sense strand with mutations in bold. Lowercase letters represent bases altered from the wild-type sequence.