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. Author manuscript; available in PMC: 2006 Oct 2.
Published in final edited form as: Biol Reprod. 2000 Mar;62(3):670–679. doi: 10.1095/biolreprod62.3.670

FIG. 4.

FIG. 4

An E box and a CCAAT box were important for transcription of SF-1 in Sertoli and Leydig cells. Six block-replacement mutants spanning the region between −83 and −51 bp of the SF-1 promoter were generated and placed into the context of the SF-1 (−734/+60) Luc vector. The sequences of the regions mutated and the respective replacement sequences (lowercase) are shown at the bottom of the figure. Mutations 1 and 4 contain sequences for E box and CCAAT box elements, respectively. Promoter activity was determined in primary rat Sertoli cells, MSC-1 cells, and MA-10 cells by transient transfection analysis as described in the legend to Figure 3. The data represent the firefly/Renilla luciferase activity of each construct normalized to the firefly/Renilla luciferase activity of the wild-type −734/+60 promoter construct. Transfections were done a minimum of three times. Error bars represent the SEM.