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. Author manuscript; available in PMC: 2006 Oct 2.
Published in final edited form as: Biochemistry. 2004 Jul 20;43(28):9234–9242. doi: 10.1021/bi049468v

Figure 4. Effect of temperature, cardiolipin and substrate on the specific activity of purified seHAS.

Figure 4

Purified seHAS was pre-incubated at pH 7.0 at temperatures ranging from 4°C to 45°C, as indicated, in the absence (Panel A, open symbols) or presence (Panel B, closed symbols) of 2 mM bovine CL plus 1 mM UDP-GlcUA for one hour. The control (•), which is shown for comparison in both A and B, was incubated at 4°C in the presence of bovine CL and UDP-GlcUA. The pre-incubated samples were then incubated with radiolabeled substrates and HAS activity was assessed at the indicated times at 30°C as described in Methods. Panel C shows the effect of CL plus UDP-GlcUA on the specific activity of purified seHAS at the indicated temperatures, as determined from the slopes of lines in A (○) or B (•).