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. Author manuscript; available in PMC: 2006 Oct 2.
Published in final edited form as: Biochemistry. 2004 Jul 20;43(28):9234–9242. doi: 10.1021/bi049468v

Figure 6. Effect of ionic strength on seHAS activity.

Figure 6

The effect of KCl and NaCl on purified seHAS was determined using the normal assay conditions to assess HAS activity described in Methods, except that two buffer concentrations were used: 25 mM KHPO4 (○,•) and 50 mM KHPO4 (□,▪) with varying NaCl (○,□) and KCl (•,▪) concentrations from 0–1.0 M. Kinetics were linear with respect to time and enzyme concentration under all conditions.