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. 2006 Oct 2;103(41):15038–15043. doi: 10.1073/pnas.0601945103

Fig. 1.

Fig. 1.

HO-Mito-Etd+ has a selective excitation wavelength at 396 nm. (a) The fluorescence emission of an equimolar concentration of Mito-Etd+ (—) overlapped 40% of the fluorescence emission of HO-Mito-Etd+ (− − −) exciting at 510 nm. (b) The excitation spectrum (emission at 579 nm) revealed HO-Mito-Etd+ (− − −) has a distinct excitation at 396 nm that is not present for Mito-Etd+ (—). (c) Excitation at 396 nm enhanced the fluorescence emission of HO-Mito-Etd+ (− − −) by 70% and reduced spectral overlap from Mito-Etd+ (—) to 10%, as compared with 510-nm excitation.