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Mito-HE oxidized by xanthine/xanthine oxidase was analyzed by electrospray ionization and ion trap mass spectrometry. The ions at m/z = 316.0 and 630.5 were identified as Mito-Etd+. The doubly charged ion at m/z = 324.0 corresponded to the molecular weight of the hypothesized structure of HO-Mito-Etd+. The singly charged ion at m/z = 646.0 lead to the proposal of the carbonyl structure, O=Mito-Etd+.
