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. 2006 Oct 3;103(41):15136–15141. doi: 10.1073/pnas.0607181103

Fig. 1.

Fig. 1.

Interaction of IRF1 with MyD88. (a) HEK293T cells were transfected with expression plasmids for IRF1YFP and MyD88CFP. Representative confocal images are shown. The arrows indicate colocalization of IRF1 with MyD88. (b and c) YFP and CFP images of HEK293T cells coexpressing IRFsYFP with MyD88CFP were obtained by using a fluorescence microscope. FRETC values were calculated and demonstrated by using a pseudocolor image (b) or FRETC/CFP values (c). (d) Lysates prepared from HEK293T cells transiently transfected with a combination of FLAG–MyD88 and HA–IRF1 or HA–IRF2 were immunoprecipitated (IP) with the anti-FLAG antibody and subjected to immunoblot (IB) analysis using the anti-HA or anti-FLAG antibody, as indicated. WCL, whole-cell extracts. (e) A schematic diagram of MyD88 truncated mutants is shown. Each FLAG–MyD88 mutant was coexpressed with HA–IRF1 in HEK293T cells and subjected to coimmunoprecipitation analysis.